Preparative Gradient Chromatography

Preparative Supercritical Fluid Chromatography

Supercritical Suid chromatography (SFC) on a preparative scale is of interest because of its advantages over high performance liquid chromatography (HPLC). The high diffusivity and low viscosity of supercritical Suids allow rapid separation. The solvent strength can easily be controlled by changing pressure and/or temperature, so that mobile phases with a Rxed composition can be used to separat...

Preparative Density Gradient Centrifugations

Large-Scale, Preparative Paper Chromatography

Most of the techniques that have been described for large-scale, preparative paper c hromatograp hy depe nd on an i.ncrease in bulk through the use of many closely pressed layers of a thin paper [IJ.' So me of these methods require the tedious loading of each s hee t , and others tend to suffer from a lack of uniformity in the movement of compounds thro ughout the bulk. Th e latter disadvantage...

Preparative Thin-Layer (Planar) Chromatography

The various OPLC andRPC techniques are compared in Table 1. Study of the data shows that OPLC is an excellent technique for analytical separations and that RPC is more ideally suited as a preparative method for isolation of compounds from biological matrices. The advantage of combining online and ofSine separations and two-dimensional development can also be exploited in OPLC. The advantage of ...

Choice of Model for Estimation of Adsorption Isotherm Parameters in Gradient Elution Preparative Liquid Chromatography

The inverse method is a numerical method for fast estimation of adsorption isotherm parameters directly from a few overloaded elution profiles and it was recently extended to adsorption isotherm acquisition in gradient elution conditions. However, the inverse method in gradient elution is cumbersome due to the complex adsorption isotherm models found in gradient elution. In this case, physicoch...